Effect of nm23-H1 on reversing malignant phenotype on human lung cancer cell line L9981.
- Author:
Guowei CHE
1
;
Qinghua ZHOU
;
Yanping WANG
;
Lunxu LIU
;
Yang QIN
;
Zilin SUN
;
Zefang SUN
;
Xiaohe CHEN
Author Information
1. The Key Laboratory of Lung Cancer Molecular Biology, West China Hospital, Sichuan University, Chengdu 610041, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Cell Line, Tumor;
Cell Proliferation;
Humans;
Lung Neoplasms;
pathology;
Mice;
Mice, Nude;
NM23 Nucleoside Diphosphate Kinases;
genetics;
Neoplasm Metastasis;
Neoplasm Transplantation;
Recombinant Fusion Proteins;
genetics;
Transfection;
Tumor Suppressor Proteins;
genetics
- From:
Journal of Biomedical Engineering
2005;22(3):530-534
- CountryChina
- Language:Chinese
-
Abstract:
nm23-H1 is a proven tumor metastasis suppressive gene, tumor metastasis phenotype could be reversed by transfected nm23-H1 cDNA. This study was conducted to transfect nm23-H1 cDNA into L9981 cells and to explore the function of nm23-H1 in reversing the malignant phenotype of L9981 cells. The plasmid of pLXSN-nm23-H1-EGFP was constructed by gene clone technique, and the transfected nm23-H1 cDNA cell lines of L9981-nm23-H1 was established. The protein expression of nm23-H1 was detected by Western blot. The biologic features of L9981-nm23-H1 cells were studied in vitro and in vivo. The results showed that the fusion protein of nm23-H1-EGFP was stable, continuous and expressed with high efficiency in L9981-nm23-H1 cells. The cell proliferation, colon formation and invasive ability are significantly lowered in L9981 cells transfected nm23-H1 cDNA (P < 0.01); the tumorgenesis and the lung metastasis incidence was lower in tranfected nm23-H1 cells than in L9981 and L9981-Plxsn in nude mice (P < 0.01); the rate for inhibiting tumorgenesis of nm23 -H1 was 82.56%. These data suggest that the malignant phenotype could be reversed by wild nm23-H1 gene in L9981 cells.
