Preparation of 5-fluorouracil-peanut agglutinin conjugate and determination of the ratio of drug to conjugate.
- Author:
Qin CAI
1
;
Yanfa MENG
;
Zhirong ZHANG
Author Information
1. West China Pharmacy College of Sichuan University, Chengdu 610041, China.
- Publication Type:Journal Article
- MeSH:
Antineoplastic Agents;
chemistry;
Cross-Linking Reagents;
chemistry;
Fluorouracil;
chemistry;
Peanut Agglutinin;
chemistry
- From:
Journal of Biomedical Engineering
2005;22(3):545-547
- CountryChina
- Language:Chinese
-
Abstract:
5-fluorouracil was combined with peanut agglutinin by a water-soluble carbodiimide to prepare the tumor target conjugate of 5-fluorouracil-peanut agglutinin and the ratio of drug to conjugate was determined by the modified trinitrobenzenesulfonic acid method (TNBS). The ratio of drug to conjugate was 76.33%. The result showed that 5-fluorouracil could link to the peanut agglutinin by EDC/NHS crosslinking with high drug ratio.
