Production of polyclonal antibody against HBD-2 by immunization with recombinant GST-HBD-2 fusion protein.
- Author:
Guoxing WANG
1
;
Yun FENG
;
Qi WU
;
Ronghua YAN
;
Xun LI
;
Boyao WANG
;
Ning HUANG
Author Information
1. Research Unit of Infection & Immunity, West China Medical Center, Sichuan University, Chengdu 610041, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Antibodies;
isolation & purification;
metabolism;
Blotting, Western;
Enzyme-Linked Immunosorbent Assay;
Escherichia coli;
metabolism;
Immunization;
Rabbits;
Recombinant Fusion Proteins;
immunology;
beta-Defensins;
immunology
- From:
Journal of Biomedical Engineering
2005;22(3):575-579
- CountryChina
- Language:Chinese
-
Abstract:
For the purpose of detecting the HBD-2 expression at protein level, the recombinant prokaryotic expression vector pGEX-1lambdaT-HBD-2 was constructed and the E. coli-based product of GST-HBD-2 fusion protein was prepared. When rabbit was immunized with the fusion protein, the anti-serum against HBD-2 was produced. After caprylic acid and ammonium sulfate precipitation, high titer of specific polyclonal antibody against HBD-2, which was detected by ELISA and Western blot, was obtained. This result suggests that recombinant peptide fusion protein could be used instead of the conjugate of peptide-albumin or peptide-thyroid globulin to produce antibody. The obtained antibodies could be used for revealing the tissue distribution of HBD-2 and the regulation of its gene expression.
