Protective effects of puerarin against 1-methyl-4-phenylpyridinium-induced mitochondrial apoptotic death in differentiated SH-SY5Y cells.
- Author:
Yuefa CHENG
1
;
Guoqi ZHU
;
Yali GUAN
;
Yingshuo LIU
;
Yan HU
;
Qinglin LI
Author Information
1. Jitang College of Hebei United University, Tangshan 063000, China. arthurcyf@163.com
- Publication Type:Journal Article
- MeSH:
1-Methyl-4-phenylpyridinium;
pharmacology;
Apoptosis;
drug effects;
Blotting, Western;
Caspase 3;
metabolism;
Caspase 8;
metabolism;
Caspase 9;
metabolism;
Cell Cycle;
drug effects;
Cell Differentiation;
drug effects;
Cell Line, Tumor;
Enzyme-Linked Immunosorbent Assay;
Flow Cytometry;
Humans;
Isoflavones;
pharmacology;
Membrane Potential, Mitochondrial;
drug effects;
Mitochondria;
drug effects
- From:
China Journal of Chinese Materia Medica
2011;36(9):1222-1226
- CountryChina
- Language:Chinese
-
Abstract:
It is well known that puerarin possesses protective activity on neurodegenerative diseases. However, the exact path way involved in the protective effect of puerarin on MPP+ -induced cell death is unclear. In this study, we focused on mitochondria im pairment in the apoptotic process of MPP+ -elicited SH-SY5Y cells and detected the protection of puerarin. As evidenced by Trypan blue assay, the cell viability was significantly decreased by 1 mmol x L(-1) MPP+, but reversed by different concentrations puerarin pre treatment. Flow cytometer analysis revealed that MPP+ -induced SH-SY5Y cells apoptosis and arrested the cells in G2/M phase, where as puerarin pretreatment concentration dependently reversed the apoptosis ratio. In addition to the apoptosis ratio, 50.0 micromol x L(-1) puerarin pretreatment even altered the MPP+ -induced G2/M phase arrest. JC-1 assay suggested that MPP+ significantly opened MMP of the SH-SYSY cells; pretreatment with puerarin attenuated the deterioration of the MMP. Both ELISA and Western blotting showed that puerarin prevented the release of cytochrome c from the mitochondrial interior to the cystol elicited by MPP+. DNA ladder showed that typical DNA ladder was present in the MPP+ -induced SH-SY5Y cells. Additionally, MPP+ enhanced caspase-9 and caspase-3 ac tivity, respectively, while not caspase-8. However,the enhancement was concentration dependently blocked by puerarin pretreatment. Taken together, puerarin can modulate mitochondrial membrane potential and inhibit the cytochrome c releasing-caspase cascade to pre vent MPP+ -induced cell injury.
