Influences of Oxytropis falcata on proliferation of SMMC-7721 hepatocarcinoma cells and expression of MMP-2.
- Author:
Guangming YANG
1
;
Fangfang ZHANG
;
Dong WANG
;
Yuanyuan HE
;
Li TONG
;
Baochangi CAI
Author Information
- Publication Type:Journal Article
- MeSH: Antineoplastic Agents, Phytogenic; pharmacology; Carcinoma, Hepatocellular; metabolism; Cell Line, Tumor; Cell Proliferation; drug effects; Drugs, Chinese Herbal; pharmacology; Enzyme-Linked Immunosorbent Assay; Humans; Matrix Metalloproteinase 2; metabolism; Oxytropis; chemistry; Polymerase Chain Reaction
- From: China Journal of Chinese Materia Medica 2011;36(9):1227-1230
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the influences of extracts of Oxytropis falcata on proliferation of SMMC-7721 cells and expression of MMP-2 (matrix metalloproteinase-2).
METHODSMMC-7721 cells were treated for 24 h with five fractions obtained from 0. falcata in different concentrations. Inhibition on proliferation of the SMMC-7721 cells was assessed by MT method. Secretion of MMP-2 was measured by ELISA in the supernatant of SMMC-7721 cells treated with fractions of essential oil and total flavonoids for 24 h. Transcription of mRNA of MMP-2 was detected by Real-time PCR.
RESULTIn MT assay, essential oil and total flavonoids showed potential antiproliferative activity on SMMC-7721 in a concentration dependent manner. Data of ELISA showed that fraction of essential oil suppressed secretion of MMP-2 significantly. Results of Real-time PCR indicated that both essential oil and total flavonoids restrained expression of mRNA of MMP-2.
CONCLUSIONIt suggested that essential oil and total flavonoids of O. falcata inhibit proliferation of SMMC-7721 cells through down-regulating secretion and expression of MMP-2 in cells. However, further experiments are necessary to carry out to investigate the potential mechanism.
