Determination of total content of ten ginsenosides in yiqifumai lyophilized injection by near infrared spectroscopy.
- Author:
Xiaoping HAN
1
;
Dekun LI
;
Dazheng ZHOU
;
Ruichao LIN
;
Lifang LIU
;
Zhengliang YE
Author Information
- Publication Type:Journal Article
- MeSH: Freeze Drying; Ginsenosides; chemistry; Spectroscopy, Near-Infrared; methods
- From: China Journal of Chinese Materia Medica 2011;36(12):1603-1605
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo determine the total content of 10 ginsenosides in Yiqifumai lyophilized injection by near infrared spectroscopy.
METHODSixty samples were collected and determined of the total contents of ten ginsenosides by HPLC. The optimal calibration model was established by the contents of 10 ginsenosides in fifty samples and their NIR spectroscopy using the PLS. And the contents of 10 samples were successfully predicted.
RESULTWhen using the pretreatment of the first derivative and MSC in the range of 4 246.8 - 4 602.2, 5 446.8 - 61 02.6 cm(-1), the best dimension was 9, and the quantitative model was accurate. The R2 was 94.2, and the RMSECV was 0.186. The RMSEP of ten samples was 0.234.
CONCLUSIONThis method is easy, rapaid and precise, and can be used to determine the content of 10 ginsenosides in Yiqifumai lyophilized injection.
