Analysis of flavonoids in platycladi cacumen by UPLC-MS.
- Author:
Mingqiu SHAN
1
;
Wen QIAN
;
Jing GAO
;
Yumei CHI
;
Li ZHANG
;
Anwei DING
Author Information
- Publication Type:Journal Article
- MeSH: Chromatography, Liquid; methods; Cupressaceae; chemistry; Flavonoids; chemistry; Mass Spectrometry; methods; Plant Extracts; chemistry
- From: China Journal of Chinese Materia Medica 2011;36(12):1626-1629
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the flavonoids in Platycladi Cacumen.
METHODThe constituents in Platycladi Cacumen were determined by UPLC-MS. A Waters BEH C18 column (2.1 mm x 150 mm, 1.7 microm) was used with a gradient elution of methanol-water containing 0.2% formic acid. The mass spectrometer equipped with electrospay ionization source was used as defector and operated in data was collected under the negative ion modes.
RESULTEleven constituents were identified.
CONCLUSIONIn this study, the main flavonoids in Platycladi Cacumen were separated by UPLC, and identified through the information of mass number and UV spectra. With the information of MS/MS from flavonoids, the modes of breaking up flavonoids were discussed. It is an accurate and effective method which can be applied for the constituent identification of Platycladi Cacumen.
