Simultaneous determination of three steroidal alkaloids from Solanum Nigrum by RP-HPLC.
- Author:
Haijian YUAN
1
;
Yigang CHEN
;
Baochang CAI
;
Xiaobin JIA
;
Yan CHEN
Author Information
- Publication Type:Journal Article
- MeSH: Alkaloids; chemistry; Chromatography, High Pressure Liquid; methods; Phytosterols; chemistry; Solanaceous Alkaloids; chemistry; Solanum nigrum; chemistry
- From: China Journal of Chinese Materia Medica 2011;36(12):1630-1632
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVEA new method for simultaneous determination of solasonine (1), solamargine (2) and khasianine (3) in Solanum Nigrum by reversed-phase HPLC was developed.
METHODThe samples were separated at 30 degrees C on Agilent Zorbax SB C18 (4.6 mm x 150 mm, 5 microm) column with acetonitrile-water-phosphoric as mobile phase. Flow rate was 1.0 mL x min(-1) and the detection wavelength was 205 nm.
RESULTThere was good linearity between the peak area and concentration at the ranges of 0.860-10.320 microg (r = 0.999 7), 0.726-8.710 microg (r = 0.999 7), 0.856-10.270 microg (r = 0.999 7) for 1, 2 and 3 respectively. The average recoveries of 1, 2 and 3 were 101.04%, 99.65%, 100.17%.
CONCLUSIONThe method is rapid, simple and accurate, and it can be used for the evaluation of Solanum Nigrum L.
