Studies on separation, purification and structure characteristics of a polysaccharide LTC-II from Pyrola corbieri.
- Author:
Zhengchang MO
1
;
Lanfang WU
;
Juan YANG
;
Daoping WANG
Author Information
- Publication Type:Journal Article
- MeSH: Chromatography; Polysaccharides; chemistry; Pyrola; chemistry
- From: China Journal of Chinese Materia Medica 2011;36(12):1633-1636
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo characterize the structure of polysaccharide LTC-II obtained from Pyrola corbieri.
METHODThe polysaccharide was extracted from P. corbieri by hot water and ethanol precipitation. Crude polysaccharide was purified by DEAE-Cellulose chromatography and Sephacryl S-300 HR column chromatography. The purity and molecular weight of polysaccharide was determined by gel permeation chromatography. UV, IR, optical rotation, complete acid hydrolysis, periodate oxydation, Smith degradation, partial acid hydrolysis and methylation analysis were applied to determine the structural features.
RESULTA homogeneous fraction LTC-II was obtained and its relative molecular mass was 22 000 Da. It consisted of arabinose, mannose, glucose, galactose in the molar ratio of 35. 2: 1.0: 13. 4: 4. 2. LTC-II had a backbone consisting glucose, mannose, galactose and mainly contained (1 --> 6)-linkaged glucose. The side chain possessed arabinose, glucose, galactose and mainly contained (1 --> 5)-linkaged arabinose. The terminal sugar were mainly glucose and galactose.
CONCLUSIONStudies on the preliminary characterization of polysaccharide LTC-II from P. corbieri for the first time.
