Analysis and identification of glycosides in Paeonia lactiflora by UPLC-MS/MS.
- Author:
Minxia ZHENG
1
;
Zhe CHEN
;
Pei LIU
;
Yuqing GE
;
Qun DAI
Author Information
- Publication Type:Journal Article
- MeSH: Molecular Structure; Paeonia; chemistry; Reproducibility of Results; Spectrometry, Mass, Electrospray Ionization; methods; Tandem Mass Spectrometry; methods
- From: China Journal of Chinese Materia Medica 2011;36(12):1641-1643
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the monoterpene glycosides in Paeonia lactflora by UPLC-MS/MS.
METHODAn Acquity UPLC BEH C18 column (2.1 mm x 50 mm) with 1.7 microm particle size was used. The mobile phase was composed of acetonitrile and 0.1% formic acid in gradient mode. The flow rate was 0.4 mL x min and the chromatographic run time was 9 min for one run. The mass spectrometer equipped with an eletrospray ion source in negative ion mode.
RESULTSTotally six glycosides were analyzed and identified by the established UPLC-MS/MS method.
CONCLUSIONThe method was rapid, sensitive, and extremely useful for rapid identification of glycosides in P. lactiflora.
