Investigation of inflammasome during excitation of IgG-HepG2 cells for evaluation of allergenic ingredients.
- Author:
Xi LU
1
;
Mingwei WANG
;
Fan LEI
;
Yugang WANG
;
Zhiyi YUAN
;
Shuang ZHAO
;
Huiyu LI
;
Dongming XING
;
Lijun DU
Author Information
- Publication Type:Journal Article
- MeSH: Allergens; analysis; immunology; Drugs, Chinese Herbal; chemistry; Gene Expression Regulation; immunology; Hep G2 Cells; Humans; Immunity, Innate; immunology; Immunoglobulin G; genetics; Inflammasomes; immunology; Promoter Regions, Genetic; genetics; Transfection
- From: China Journal of Chinese Materia Medica 2011;36(14):1855-1859
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the alteration of inflammasome and receptor during IgG promoter transfected to HepG2 cells.
METHODBy assay of Elisa to evaluate the secretion of IL-1 beta, IL-8, TNF-alpha and MCP-1 after puerarine and LPS administration, and by assay of real time PCR to evaluate the expression of mRNA of IL-1 beta, IL-8,TNF-alpha and MCP-1, as well as the receptors of TLR2, 4 and NOD2, MyD88.
RESULTIgG promoter did not active innate immunity and enhance the expression and secretion of inflammasome in HepG2. Puerarine did not active the inflammasome either. LPS activated the innate immunity and increased the secretion of IL-8, TNF-alpha and MCP-1.
CONCLUSIONIgG-HepG2 cells could be used specifically as the model of allergy type II for ingredients screening. It is suggested that puerarine was suite for the activator for this type of allergy as positive control.
