Effect of Qingkailing injection (QKLI) on complement and RBL-2 H3 cells in virto.
- Author:
Lijing CHEN
1
;
Guoping LIAO
;
Yan WANG
;
Shuai HE
;
Zhongyi ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Adolescent; Adult; Animals; Cell Degranulation; drug effects; Cell Line, Tumor; Chemistry, Pharmaceutical; Complement System Proteins; metabolism; Drugs, Chinese Herbal; administration & dosage; chemistry; pharmacology; Hexosaminidases; secretion; Histamine Release; drug effects; Humans; Injections; Rats; Young Adult
- From: China Journal of Chinese Materia Medica 2011;36(14):1884-1888
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effect of Qingkailing injection (QKLI) on complement and RBL-2 H3 cells in virto.
METHODThe mixture of human serums and QKLI were incubated for 30 min in vitro and then the content of SC5 b-9 in the mixture was determined by ELISA. RBL-2 H3 cells were cultured and treated by QKLI. Beta-heosaminidase release rate was measured by coloration method. The content of histamine in supernatant was tested by ELISA.
RESULTThe QKLI can reduce the content of SC5 b-9 (P<0.05) and promote the release of beta-heosaminidase and histamine significantly (P<0.05).
CONCLUSIONQKLI didn't induce the complement activation, but induced the release of beta-heosaminidase and histamine directly. Therefore, the clinical adverse reactions of QKLI in clinic may be pseudoallergy which had no relation with the activation of complement system.
