Study on correlation between ITS sequence of Arctium lappa and quality of Fructus Arctii.
- Author:
Liang XU
1
;
Deqiang DOU
;
Bing WANG
;
Yanyun YANG
;
Tingguo KANG
Author Information
- Publication Type:Journal Article
- MeSH: Arctium; chemistry; genetics; Computational Biology; DNA, Ribosomal Spacer; genetics; Drugs, Chinese Herbal; standards; Furans; analysis; Genotype; Glucosides; analysis; Polymorphism, Genetic; genetics; Quality Control; Sequence Analysis, DNA; Software
- From: China Journal of Chinese Materia Medica 2011;36(14):1931-1935
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the correlation between ITS sequence of Arctium lappa and Fructus Arctii quality of different origin.
METHODThe samples of Fructu arctii materials were collected from 26 different producing areas. Their ITS sequence were determined after polymerase chain reaction (PCR) and quality were evaluated through the determination of arctiin content by HPLC. Genetic diversity, genotype and correlation were analyzed by ClustalX (1.81), Mage 4.0, SPSS 13.0 statistical software.
RESULTITS sequence of A. was obtained from 26 samples, and was registered in the GenBank. Corresponding arctiin content of Fructus arctii and 1000-grain weight were determined. A. lappa genotype correlated with Fructus arctii quality by statistical analysis.
CONCLUSIONThe research provided a foundation for revealing the molecular mechanism of Fructus arctii geoherbs.
