Study on in vitro microdialysis recovery of Shuanghuanglian.
- Author:
Xiang FU
1
;
Jiajun LING
;
Xiujun WU
Author Information
- Publication Type:Journal Article
- MeSH: Calibration; Chromatography, High Pressure Liquid; Drugs, Chinese Herbal; analysis; isolation & purification; pharmacokinetics; Feasibility Studies; Microdialysis; methods
- From: China Journal of Chinese Materia Medica 2011;36(14):1951-1954
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the feasibility of microdialysis techniqiue to be used in pharmacokinetic study of Chinese medicine, taking Shuanghuanglian as a model drug.
METHODThe samples were obtained by retrodialysis, determined by HPLC gradient elution to calculate the in vitro recovery rate (RR) of specific components. To study the difference of RR of a certain component in different dialysis mediums, and the effect of flow rates and concentration on RR.
RESULTAlong with the increase of number of substances in the dialysis medium, the RR of specific components reduced. But the RR was independent of the concentration of the component in the dialysis medium. The RR reduced with the increasing flow rate in the same dialysis medium.
CONCLUSIONThe microdialysis technique can be used in pharmacokinetics study of Chinese medicine.
