Study on methodology of determination of Yizhi osmotic pump tablets active ingredient in Beagle dog plasma by LC-MS/MS.
- Author:
Yanming WANG
1
;
Shouying DU
;
Yongsong ZHAI
;
Yang LU
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Blood Chemical Analysis; methods; Calibration; Chromatography, Liquid; methods; Dogs; Drug Stability; Drugs, Chinese Herbal; analysis; pharmacokinetics; Male; Osmosis; Tablets; Tandem Mass Spectrometry; methods; Temperature; Time Factors
- From: China Journal of Chinese Materia Medica 2011;36(14):1964-1968
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo develop a sensitive and specific LC-MS/MS method for determination of Yizhi osmotic pump tablets active ingredient in Beagle dog plasma.
METHODBeagle dog plasma pre-treatment methods were established. Geniposide, notoginsenoside R1, ginsenoside Rg1 and Rb1 notoginsenoside molecular ions and fragment ions peaks were separated well and detected synchronously by LC-MS/MS with digoxin as internal standard.
RESULTUnder the selected LC-MS/MS conditions, the characteristic fragment ions of the four components could be well separated and quantified, and the calibration curves showed good linearity within a certain concentration range of each component; extraction recoveries of those four compounds in plasma were higher than 75%, method recoveries were higher than 90%; day precision (RSD <10%) and inter-day precision (RSD <15%) were generally fine; freeze-thaw and room temperature experiments showed good stability (RSD <15%).
CONCLUSIONThe method is proved to be suitable for pharmacokinetic studies of Yizhi active ingredients in Beagle dog plasma.
