Quantitative determination of contents of three components in Brucea javanica by HPLC.
- Author:
Zhongliu ZHOU
1
;
Renbing SHI
;
Bin LIU
;
Jieming ZOU
;
Lisheng WANG
;
Jingmin XIA
Author Information
- Publication Type:Journal Article
- MeSH: Brucea; chemistry; Chromatography, High Pressure Liquid; methods; Drugs, Chinese Herbal; analysis; isolation & purification; standards; Linear Models; Organic Chemicals; analysis; isolation & purification; standards; Quality Control
- From: China Journal of Chinese Materia Medica 2011;36(14):1979-1981
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo develop an HPLC method for quantitative determination of three quassinoids in Brucea javanic.
METHODThe determination was carried out on a phenomenex C18 column (4.6 mm x 250 mm, 5 microm) with gradient elution program of methol-water at a flow rate of 1.0 mL x min(-1), and the detection wavelength was 270 nm.
RESULTLinearites of bruceine D, brusatol and bruceine H were good (r = 0.9996, 0.9996, and 0.9998) in ranges of 2.52-12.60, 2.19-10.95, and 2.91-14.55 microg, respectively. The average recoveries of bruceine D, brusatol and bruceine H were 100.01%, 100.95% and 100.43% respectively, and RSD of the above three compounds were 0.31% (n = 6), 1.7% ( n = 6) and 1.7% (n = 6), respectively.
CONCLUSIONThe determination results of three batch samples showed that the method was simple, accurate and could be used in the quantitative determination of three components in the B. javanica.
