Determination of astragaloside IV of eight area in Astragali Radix is by HPLC-ELSD internal standard method.
- Author:
Caiyun PEI
1
;
Zongquan WANG
;
Jiming JA
;
Jian SONG
Author Information
- Publication Type:Journal Article
- MeSH: Astragalus Plant; chemistry; Chromatography, High Pressure Liquid; standards; Drugs, Chinese Herbal; analysis; Linear Models; Reference Standards; Saponins; analysis; Triterpenes; analysis
- From: China Journal of Chinese Materia Medica 2011;36(14):1982-1984
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish an HPLC-ELSD internal standard method for determination of astragaloside IV in Astragali Radix.
METHODWith Ginsenoside Rb2 as internal standard, the separation were carried out on an Agilent TC-C18 (4.6 mm x 150 mm, 3.5 microm) column with methanol-water (72: 28) as mobile phase. The flow rate was 1.0 mL x min(-1) and the drift tube temperature of the ELSD was 75 degrees C. The gas pressure was set at 172.4 kPa using the clean and dry compressed air as spray gas.
RESULTThere was good linearity in the range of 0.5624-5.624 microg of astragaloside IV (r = 0.9999); The average recovery was 98.06% with RSD of 0. 98%.
CONCLUSIONThe internal standard method is accurate and reproducible, and suitable for quality control of radix astragali.
