Correlation between H2O2 scavenging system and flavonoids accumulation of Scutellaria baicalensis.
- Author:
Lingfei SHUAI
1
;
Yuan YUAN
;
Ping CHEN
;
Shufang LIN
Author Information
- Publication Type:Journal Article
- MeSH: Anti-Infective Agents; analysis; metabolism; Antioxidants; analysis; metabolism; Cells, Cultured; Darkness; Flavanones; analysis; metabolism; Flavonoids; analysis; metabolism; Hot Temperature; Hydrogen Peroxide; analysis; metabolism; Oxidants; metabolism; Oxidative Stress; Peroxidase; metabolism; Phenylalanine Ammonia-Lyase; metabolism; Plant Extracts; chemistry; Plant Roots; chemistry; drug effects; enzymology; metabolism; Plants, Medicinal; Polyethylene Glycols; pharmacology; Scutellaria baicalensis; chemistry; drug effects; enzymology; metabolism; Stress, Physiological
- From: China Journal of Chinese Materia Medica 2011;36(13):1707-1710
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVEStudy on correlation between H2O2 scavenging system and flavonoids accumulation of Scutellaria baicalensis.
METHODThe content of baicalin and baicalein in suspension cell of S. baicalensis was determined by HPLC. The content of total flavonoids and H2O2, the activity of POD and PAL was detected by UV spectrophotometry.
RESULTThe content of total flavonoid and the activity of PAL increased significantly in 12 days after 40 degrees C, dark and PEG stress. Around 12 days after NPA, NPA +40 degrees C, 40 degrees C, NPA + dark, dark and PEG stress, the content of baicalin declined and the content of baicalein rise, the activity of POD showed an increasing trend, and level of H2O2 remain stable.
CONCLUSIONModerate environmental stress could promote the accumulation of total flavonoids in S. baicalensis, baicalin convert to baicalein by POD, and maintaining the stability of H2O2 content to avoid oxidative damage.
