Influence of penetration enhancers on in vitro transdermal permeation of L-tethrahydropalmatine.
- Author:
Li QIAN
1
;
Zhen MA
;
Wanggang ZHANG
;
Qiao WANG
Author Information
- Publication Type:Journal Article
- MeSH: Administration, Cutaneous; Animals; Azepines; pharmacology; Berberine Alkaloids; analysis; pharmacokinetics; Drug Synergism; Eucalyptus; chemistry; Male; Menthol; pharmacology; Oils, Volatile; pharmacology; Permeability; drug effects; Rats; Rats, Sprague-Dawley; Skin; drug effects; metabolism; Skin Absorption; drug effects
- From: China Journal of Chinese Materia Medica 2011;36(13):1729-1732
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the effect of different penetration enhancers on the in vitro transdermal permeation of 1-tethrahydropalmatine (L-THP) through rat skin.
METHODBoth natural and chemical synthesis penetration enhancers were applied singly or jointly to investigate the skin permeation rates of l-THP. The skin permeation profiles were evaluated by Valian-Chien permeation cells with isolated rat skin. HPLC-UV method was established to determine the concentration of l-THP in samples.
RESULTAs chemical synthesis penetration enhancer was used alone, 8% azone was observed to be the optimal penetration enhancer with the maximal penetration rate of 21.153 microg x cm(-20 x h(-1). Although 2% menthol crystal or 5% eucalyptus oil was effective as a natural penetration enhancer when used alone, the average penetration rate reached only half of that of 8% azone. The penetration potency of either menthol oil or menthol crystal combined with 8% azone was more effective than that of azone alone (P < 0.05).
CONCLUSIONEither menthol oil or menthol crystal combined with 8% azone is effective on transdermal penetration of l-THP in vitro. There is significant synergistic effect when natural penetration enhancers combined with chemical synthesis penetration enhancers.