Enriching blood effect comparison in three kinds of blood deficiency model after oral administration of drug pair of Angelicae Sinensis Radix and Chuanxiong Rhizoma and each single herb.
- Author:
Weixia LI
1
;
Yuping TANG
;
Jianming GUO
;
Meiyan HUANG
;
Wei LI
;
Dawei QIAN
;
Jin'ao DUAN
Author Information
- Publication Type:Journal Article
- MeSH: Administration, Oral; Angelica sinensis; chemistry; Animals; Ca(2+) Mg(2+)-ATPase; drug effects; metabolism; Cyclophosphamide; pharmacology; Drug Therapy, Combination; Drugs, Chinese Herbal; chemistry; Erythrocytes; drug effects; enzymology; Female; Hematologic Diseases; drug therapy; etiology; Hemoglobins; drug effects; Leukocytes; drug effects; Medicine, Chinese Traditional; Mice; Models, Animal; Phenylhydrazines; pharmacology; Plant Roots; chemistry; Random Allocation; Rhizome; chemistry; Sodium-Potassium-Exchanging ATPase; drug effects; metabolism; Spleen; drug effects; immunology; Thymus Gland; drug effects; immunology
- From: China Journal of Chinese Materia Medica 2011;36(13):1808-1814
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVEThrough establishing different blood deficiency animal model, to evaluate enriching blood effect changes of the drug pair of Angelicae Sinensis Radix and Chuanxiong Rhizoma and each single herb, and to explore the effect characteristics of their compatibility.
METHODThree different methods of acetyl phenylhydrazine (APH) hemolytic method, cyclophosphamide (CTX) chemical damage method, APH-CTX complex method were used respectively to copy different blood deficiency model mice. Changes of orbit blood routine, thymus index, spleen index and ATPase activity of red cell membrane of model mice were tested.
RESULTCompared with normal group, all indexes had significant differences in three model mice. The drug pair and each single herb had significant impact on most indexes of the APH-CTX complex model mice, and on the individual indexes of APH hemolytic model mice and CTX chemical damage model mice. Therefore, APH and CTX complex blood deficiency model was more suitable for the enriching blood mechanism study of the drug pair of Angelicae Sinensis Radix and Chuanxiong Rhizoma. Compared with the single herb of Angelicae Sinensis Radix and Chuanxiong Rhizoma, the drug pair of them had presented enriching blood effect at different extent with strengthening trend in regulating the invigorating blood indexes, immune organs and energy metabolic enzymes.
CONCLUSIONThe results of this research have provided scientific basis for revealing the mutual promotive composition law of the drug pair of Angelicae Sinensis Radix and Chuanxiong Rhizoma, and responded effectively the mult-link and mult-target effect characteristics of Chinese medicine bio-effect, to offer reference for the bio-effect research of the complicated substance group of Chinese medicine and traditional Chinese medicine formulae, and to supply demonstrative reference for researching the formulae compatibility law which takes the single drug-drug pair-formulae as main line.
