Microsatellite instability and loss of heterozygosity of fragile histidine triad gene in laryngeal squamous cell carcinoma.
- Author:
De-Tao YIN
1
;
Ming-Min DONG
Author Information
- Publication Type:Journal Article
- MeSH: Acid Anhydride Hydrolases; genetics; Adult; Aged; Carcinoma, Squamous Cell; genetics; pathology; Female; Humans; Laryngeal Neoplasms; genetics; pathology; Loss of Heterozygosity; Male; Microsatellite Instability; Middle Aged; Neoplasm Proteins; genetics
- From: Chinese Journal of Otorhinolaryngology Head and Neck Surgery 2005;40(1):45-48
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the loss of heterozygosity (LOH) and microsatellite instability (MSI) of fragile histidine triad (FHIT) gene in laryngeal squamous cell carcinoma (LSCC).
METHODSUsing polymerase chain reaction-simple sequence length polymorphism (SSLP) -silver staining technique, 41 samples of LSCC were detected for LOH and MSI with microsatellite sites D3S1234 and D3S1300.
RESULTSLOH was found in 44.4% (16/36) and 36.4% (12/33) of informative cases at D3S1234 and D3S1300 respectively, while MSI at the two loci was found in 19.4% (7/36) and 24.2% (8/33) of informative cases respectively. In 41 cases of LSCC, 38 cases were informative at either locus. 52.6% (20/38) of cases had LOH while 28.9% (11/38) had MSI. The rate of LOH was related to the tumor TNM stage, pathological grade, lymph node metastasis and tumor relapse (P < 0.05). The rate of MSI was related to the tumor lymph node metastasis (P < 0.05 ).
CONCLUSIONThe frequencies of LOH and MSI in the two microsatellite sites, D3S1234 and D3S1300, of FHIT gene might play a role in carcinogenesis and development of LSCC and might provide some new approaches for early gene detection for LSCC.
