Local cytokines profile in gastric cancer lesions.
- Author:
Xiaoyin ZHANG
1
;
Yongyi CHEN
;
Yong LIU
;
Xisheng ZHOU
;
Daiming FAN
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Aged; Aged, 80 and over; CD4-Positive T-Lymphocytes; CD8-Positive T-Lymphocytes; immunology; Cytokines; metabolism; Female; Humans; Immunotherapy; Male; Middle Aged; Polymerase Chain Reaction; Stomach Neoplasms; immunology; metabolism; therapy
- From: Chinese Journal of Oncology 2002;24(1):14-16
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the characteristics of local cytokines profile in human gastric cancer lesions and offer guides for immunotherapy of gastric cancer.
METHODSThe cytokines profile in isolated CD4+, CD8+ T subsets and epithelial cells from cancer tissue, contrasted with benign gastric mucosa of 15 gastric cancer patients were analyzed by a highly sensitive radioactivity labeled semi-quantitative reverse transcription polymerase chain reaction (RT-PCR) technique.
RESULTSThe predominance of increased Th2 and reduced Th1 immune response in both CD4+ and CD8+ T subsets from cancer tissue, compared with benign gastric mucosa, were observed. IL-6, IL-8 and TNF-alpha in CD8+ T cells from cancer tissue showed statistical higher levels than those from benign gastric mucosa (P = 0.029, P = 0.022, P = 0.002), so did IL-10, TGF-beta, TNF-alpha in T cells and epithelial cells.
CONCLUSIONA shift of Th1/Th2 cytokines to Th2 is found in T cells from local tumor lesion of gastric cancer, which suggests an immune suppressive status in local tumor lesion. The obvious change of cytokine profile in CD8+ T cell suggests they play a main role in immune suppression. To study the cytokine expression of different cell subsets, we are able to offer guides for immunotherapy for patients with gastric cancer.
