Plerosis of cDNA array of normal human nasopharyngeal tissue and nasopharyngeal carcinoma.
- Author:
Weinong HAN
1
;
Hong LI
;
Lu XIE
;
Liangguo XU
;
Ling ZHANG
;
Kaitai YAO
Author Information
- Publication Type:Journal Article
- MeSH: DNA Repair; genetics; Gene Expression Profiling; Gene Expression Regulation, Neoplastic; Humans; Nasopharyngeal Neoplasms; genetics; pathology; Nasopharynx; metabolism; pathology; Oligonucleotide Array Sequence Analysis; methods; RNA, Neoplasm; genetics; Reverse Transcriptase Polymerase Chain Reaction
- From: Chinese Journal of Oncology 2002;24(2):114-117
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo compare gene expression gene profile of nasopharyngeal carcinoma (NPC) tissue with that of normal nasopharyngeal tissues by cDNA array and to discuss possible functions of DNA repair-related genes in NPC tissue.
METHODSAfter hybridization of atlas human cancer cDNA expression array 7742 - 1, atlas hybridization results were analyzed by Atlas Image 1.01 a software package. Using RT-PCR was used to confirm the results.
RESULTSOf 63 differentially expressed genes in quadrangle C including DNA damage response, repair & recombination-related genes, 6 DNA repair-related genes were up-regulated, 12 were down-regulated.
CONCLUSIONDNA repair-related genes may be involved in patho-physiological process of nasopharyngeal carcinoma.
