Action of apoptosis-induced ligand gene in relation to tumor necrosis factor on human colon cancer cell line HT29.
- Author:
Chao HE
1
;
Xiaotong HU
;
Xiangming XU
;
Bingliang FANG
Author Information
- Publication Type:Journal Article
- MeSH: Adenoviridae; genetics; Apoptosis; genetics; physiology; Apoptosis Regulatory Proteins; Cell Division; genetics; physiology; Colonic Neoplasms; genetics; pathology; therapy; Genetic Therapy; methods; Genetic Vectors; genetics; HT29 Cells; Humans; Membrane Glycoproteins; genetics; physiology; TNF-Related Apoptosis-Inducing Ligand; Transfection; Tumor Necrosis Factor-alpha; genetics; physiology
- From: Chinese Journal of Oncology 2002;24(2):133-136
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo evaluate the gene therapeutic efficiency of apoptosis-inducing ligand (TRAIL) related to tumor necrosis factor on human colon cancer cell line HT29.
METHODSHuman colon cancer cell line HT29 was transfected with adenovirus-mediated TRAIL gene Ad/GT-TRAIL. The morphological changes, cell growth and apoptosis were measured by phase contrast microscope, MTT method and flow cytometry.
RESULTSObvious morphological changes in HT29 cells was induced by Ad/GT-TRAIL and Ad/PGK-GV16. The cell suppression percentage and the percentage of apoptotic cells were 54.3% and 11.1%, respectively. When used in combination with Ad/PGK-GV16, HT29 was suppressed to 82.7% and the percentage of apoptotic cells was 24.6%. This result showed significantly enhanced therapeutic efficiency on HT29 and thus inhibiting of its growth (P < 0.05).
CONCLUSIONAd/GT-TRAIL is able to induce apoptosis of HT29 and inhibit its growth. Ad/GT-TRAIL shows significantly enhanced therapeutic efficiency for HT29 when used in combination with Ad/PGK-GV16.
