Relationship between polymorphism of N-acetyltransferase 2 and genetic susceptibility to laryngeal carcinoma.
- Author:
Dapeng LEI
1
;
Xinliang PAN
;
Chenhong GUO
;
Fenglei XU
;
Liqiang ZHANG
;
Dayu LIU
;
Xinyong LUAN
Author Information
- Publication Type:Journal Article
- MeSH: Aged; Alleles; Arylamine N-Acetyltransferase; genetics; DNA, Neoplasm; genetics; Female; Gene Frequency; Genetic Predisposition to Disease; genetics; Genotype; Humans; Laryngeal Neoplasms; genetics; pathology; Male; Middle Aged; Polymorphism, Genetic; Smoking; Statistics as Topic
- From: Chinese Journal of Oncology 2002;24(2):154-156
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the relationship between polymorphism of N-acetyltransferase (NAT2) gene and genetic susceptibility to laryngeal carcinoma.
METHODSA case-control study on 62 laryngeal carcinoma patients and 56 controls was conducted. NAT2 alleles were differentiated by polymerase chain reaction-based restriction fragment length polymorphism (PCR-RFLP) methods using originally created PCR primers and genomic DNA extracted from peripheral white blood cells. Genetic risk for NAT2 genotype was analyzed by smoking index (SI, cigarettes smoked per day x years of smoking).
RESULTSThe frequency of NAT2 slow genotype was 80.6% in patients with laryngeal carcinoma and 60.7% in the controls, the difference of which was statistically significant (chi(2) = 5.70, P = 0.017). The odds ratios were 2.70 (95% CI 1.19 approximately 6.11). Among the individuals with NAT2 slow genotype at high level of cigarette smoking, there was a significantly higher risk of 5.64 (95% CI 1.77 approximately 17.92), while those at low level were considered the reference group (OR 1.38, 95% CI 0.42 approximately 4.52).
CONCLUSIONNAT2 slow genotype increases the risk of susceptibility to laryngeal carcinoma. The combined effect of NAT2 slow genotype and exposure to smoking is observed during the development of laryngeal cancer.
