Overexpression of cyclin A in hepatocellular carcinoma and its relationship with HBx gene integration.
- Author:
Yi ZHANG
1
;
Zhihai PENG
;
Guoqiang QIU
;
Zhaowen WANG
;
Wei GU
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Aged; Carcinoma, Hepatocellular; genetics; metabolism; physiopathology; virology; Cyclin A; genetics; Female; Gene Expression; Genes, Viral; Hepatitis B virus; genetics; Humans; Liver Neoplasms; genetics; metabolism; physiopathology; virology; Male; Middle Aged; RNA, Messenger; Trans-Activators; genetics; Virus Integration
- From: Chinese Journal of Oncology 2002;24(4):353-355
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo investigate the frequency of cyclin A overexpression in hepatocellular carcinoma (HCC) and its relationship with clinical significance and HBx gene integration.
METHODSPCR, RT-PCR and Western blot were used to detect the gene, mRNA and protein level of cyclin A in the tumor and nontumorous tissue. PCR and Southern blot were used to detect the integration of HBx gene in HCC.
RESULTSAmplification of cyclin A gene was found in 1 of 35 patients; overexpression of cyclin A mRNA and protein was found in 16 of 35, 21 of 35 patients, respectively. Overexpression of cyclin A protein was correlated with patient's age, tumor size and HBx integration.
CONCLUSIONOverexpression of cyclin A occurs in the early stage of HCC carcinogenesis. It may be one of the important approaches by which HBV affects the normal cell cycle of hepatocyte.
