Cloning of adriamycin-resistant related (arr) gene in an adriamycin-resistant L1210 variant.
- Author:
Jae Ryong KIM
1
;
Seong Yong KIM
;
Jung Hye KIM
Author Information
1. Department of Biochemistry, College of Medicine, Yeungnam University, Daegu, Korea.
- Publication Type:Original Article ; Research Support, Non-U.S. Gov't
- Keywords:
MDR;
arr;
DD-PCR;
RACE
- MeSH:
Amino Acid Sequence;
Animal;
Base Sequence;
Blotting, Northern;
Cloning, Molecular;
DNA Primers;
Doxorubicin/pharmacology*;
Drug Resistance, Neoplasm/genetics*;
Gene Expression Regulation, Neoplastic;
Glycoproteins/genetics*;
Leukemia L1210/genetics*;
Leukemia L1210/drug therapy;
Mice;
Molecular Sequence Data;
Translation, Genetic
- From:Experimental & Molecular Medicine
1998;30(3):145-149
- CountryRepublic of Korea
- Language:English
-
Abstract:
A partial fragment of novel sequence (arr, adriamycin-resistant related) was previously identified using the differential display (DD)-PCR technique with adriamycin-resistant L1210 variant (L1210AdR), which shows a typical multidrug resistant (MDR) phenotypes. The present research shows the isolation of full length arr cDNA sequence. To clone the full length cDNA of arr gene, DD-PCR fragments were subjected to 5'- and 3'-Rapid Amplification of cDNA End (RACE) method. The cloned arr cDNA consisted of 770 bases and contained an open reading frame of 153 bases, encoding a protein of 51 amino acid with the molecular mass of 4 kDa by in vitro translation reactions. Northern blot analysis showed that a 770 bases transcript arr gene was overexpressed in adriamycin-resistant L1210 variant, but not in the parent suggesting that the arr gene may be involved in the adriamycin-resistant phenotypes.
