Association of RNase3 Polymorphisms with the Susceptibility of Gastric Cancer.
10.4174/jkss.2010.78.5.283
- Author:
Ja Wook KOO
1
;
Dong Baek KANG
;
Won Cheol PARK
;
Young Hwan LEE
;
In Hong KANG
;
Soo Cheon CHAE
;
Jeong Kyun LEE
Author Information
1. Department of Surgery, Institute of Medical Science, College of Medicine, Wonkwang University, Iksan, Korea. rjk@wonkwang.ac.kr
- Publication Type:Original Article
- Keywords:
RNase3;
Polymorphism;
Gastric cancer
- MeSH:
Case-Control Studies;
Eosinophils;
Gene Frequency;
Genotype;
Haplotypes;
Humans;
Immune System;
Leukocytes;
Polymorphism, Single Nucleotide;
Ribonucleases;
Stomach Neoplasms
- From:Journal of the Korean Surgical Society
2010;78(5):283-289
- CountryRepublic of Korea
- Language:Korean
-
Abstract:
PURPOSE: RNase3 is a secretory ribonuclease, which is found in the eosinophilic leukocyte and involved in the innate immune system. Its cytotoxic activity is effective against a wide range of pathogens. We performed a case-control study to examine the relationship between RNase3 polymorphisms and the susceptibility of gastric cancer in Korean people. METHODS: Blood sampling of stomach cancer and healthy persons groups were performed, Taqman in g.-550A>G, polymerase chain reaction-restriction fragment length polymorphism in g.371C>G, and high-resolution melt in g.499C>G were analyzed. The three single nucleotide polymorphisms g.-550A>G, g.371C>G, and g.499C>G in RNase3 and their haplotypes were analyzed. RESULTS: The genotype and allele frequencies of RNase3 g.-550A>G and g.371C>G were not significantly increased in susceptibility of gastric cancer than control group. But, RNase3 CC genotype was associated with a significantly increased susceptibility of gastric cancer than control group (P=0.002). Also, RNase3 CC genotype was more specifically associated with a significantly increased susceptibility of middle and lower gastric cancer than upper gastric cancer (P=0.002). In haplotype of RNase3 SNP g.-550A, g.371G, and g.499C, there was significantly susceptibility of gastric cancer (P=0.004), and more specific influence on middle and lower gastric cancer than upper gastric cancer (P=0.006 vs 0.054). CONCLUSION: RNase3 g.499C>G polymorphism may influence gastric cancers, and have a more specific influence on middle and lower gastric cancer rather than upper gastric cancer. But RNase3 g.-550A>G, g.371C>G polymorphisms need careful interpretation and confirmation in more larger studies.
