Relationship between endothelial protein C receptor gene 6936A/G polymorphisms and deep venous thrombosis.
- Author:
Xu-Dong CHEN
1
;
Lu TIAN
;
Cheng-Fei ZHENG
;
Ming LI
;
Wei JIN
;
Xiao-Hui WANG
;
Song-Ling PAN
Author Information
- Publication Type:Journal Article
- MeSH: Case-Control Studies; Humans; Polymorphism, Genetic; Protein C; metabolism; Thrombosis; Venous Thrombosis; genetics
- From: Chinese Journal of Hematology 2010;31(9):607-609
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the relationship between endothelial protein C receptor(EPCR) gene 6936A/G polymorphism and deep vein thrombosis (DVT).
METHODSThe study group included 65 DVT patients and 71 normal controls. Plasma sEPCR was measured by ELISA. Genomic DNA was extracted by using Genomic Purification Kit. A 315bp EPCR product was amplified by a standard PCR reaction, and the bands were confirmed by direct sequencing after purification.
RESULTS(1) sEPCR levels in healthy controls with 6936AG genotype were significantly higher than that in those with 6936AA genotype \[(0.97 ± 0.32) ng/L vs (0.61 ± 0.24) ng/L, P < 0.01)\], and so did in DVT patients \[(0.87 ± 0.21) ng/L vs (0.50 ± 0.18) ng/L, P < 0.01\]. (2) The sEPCR levels of DVT patients \[(0.68 ± 0.32) ng/L\] were significantly higher than that of healthy controls \[(0.54 ± 0.22) ng/L\](P < 0.05). (3) The distribution of 6936A/G genotype was higher in DVT patients than in healthy controls (P < 0.05). (4) Subjects with 6936A/G had an increased risk of thrombosis (OR = 2.75, 95%CI = 1.04 - 7.30) (P < 0.05).
CONCLUSIONSEPCR gene 6936A/G polymorphism is associated with increased plasma sEPCR levels. The sEPCR levels in DVT patients were significantly higher than that in healthy controls. The subject with 6936AG likely had an increased risk of thrombosis.
