Study on aberration in histone methylation and acetylation in acute leukemia.
- Author:
Xu-Dong MA
1
;
Yi-Qun HUANG
;
Li-Yun XIAO
;
Yong ZOU
Author Information
- Publication Type:Journal Article
- MeSH: Acetylation; DNA Methylation; Histones; metabolism; Humans; Leukemia; Methylation; Protein Processing, Post-Translational
- From: Chinese Journal of Hematology 2010;31(8):523-526
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the aberration in histone H3K4 and H3K9 methylation and H3 and H4 acetylation in human acute leukemia (AL) cells.
METHODSThe histone H3K4 and H3K9 methylation and H3 and H4 acetylation were detected by Western blot in 34 AL patients and 13 controls (9 non leukemia patients, 4 healthy volunteers).
RESULTSThe level of H3K4 methylation was significantly lower in 19 AL patients than in non leukemia (0.220 ± 0.096 vs 0.447 ± 0.186, P < 0.01), while the level of H3K9 methylation was significantly higher (0.409 ± 0.106 vs 0.168 ± 0.015, P < 0.01); Both level of histone H3 and H4 acetylation in 15 AL patients were significantly lower (H3: 0.128 ± 0.013 vs 0.386 ± 0.104, H4: 0.096 ± 0.008 vs 0.341 ± 0.096, respectively, both P < 0.01).
CONCLUSIONAberration of histone methylation and deficient histone acetylation in AL may represent the markers for an aberrant post-translational modification of histones and chromatin structure. It might be a potential epigenetic target for anti-leukemia agent.
