Effects of histone deacetylase inhibitor on the expression of angiogenesis related factors in Kasumi-1 leukemic cell line.
- Author:
Cui-Min ZHU
1
;
Zhi-Hua ZHANG
;
Feng-Yun JIANG
;
Bao-Qin LIU
;
Lei ZHAO
;
Wen-Liang TIAN
;
Li-Na YAN
;
Zhi-Qiang LIANG
;
Chang-Lai HAO
Author Information
- Publication Type:Journal Article
- MeSH: Angiogenesis Inducing Agents; Cell Line; Histone Deacetylase Inhibitors; pharmacology; Humans; RNA, Messenger; genetics; Valproic Acid; pharmacology; Vascular Endothelial Growth Factor A
- From: Chinese Journal of Hematology 2010;31(7):466-469
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effects of two histone deacetylase (HDAC) inhibitors, valproic acid (VPA) and TSA, on the expression of vascular endothelial growth factor (VEGF) and its receptor KDR of the leukemia cell line Kasumi-1 cells, and to explore their potential mechanism in leukemia angiogenesis.
METHODKasumi-1 cells were treated with VPA and TSA at different concentrations for 3 days. The mRNA and protein expression levels of VEGF and KDR were determined by semi-quantitative RT-PCR and Western blot, and the bFGF mRNA by semi-quantitative RT-PCR.
RESULTSAs compared with that of control groups, VPA at 3 mmol/L downregulated the VEGF mRNA expression level for VEGF(121) from 0.632 ± 0.014 to 0.034 ± 0.004 and for VEGF(165) from 0.526 ± 0.021 to 0.015 ± 0.001, for KDR mRNA from 0.258 ± 0.034 to 0.038 ± 0.000, and for bFGF mRNA from 0.228 ± 0.017 to 0.086 ± 0.015. TSA downregulated the VEGF mRNA and KDR mRNA at concentration of 100 nmol/L, but its effect on bFGF mRNA only at higher concentration.
CONCLUSIONHDAC inhibitors might inhibit the leukemia angiogenesis by regulating the expression of VEGF and its recptor.