Effect of astragalus polysaccharide on the function and maturation of plasmacytoid dendritic cells from chronic myelogenous leukemia before and after treatment.
- Author:
Li-Min LIU
1
;
Lian-Sheng ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Cells, Cultured; Dendritic Cells; immunology; Humans; Interferon-alpha; pharmacology; Leukemia, Myelogenous, Chronic, BCR-ABL Positive; immunology; Leukocytes, Mononuclear; Polysaccharides; pharmacology
- From: Chinese Journal of Hematology 2010;31(11):740-743
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the effect of astragalus polysaccharide (APS) on the function and maturation of chronic myelogenous leukemia (CML) peripheral blood mononuclear cells (PBMC)-derived plasmacytoid dendritic cells (pDCs).
METHODSCML-derived pDCs were sorted by flow cytometry, and then incubated with APS (at 0, 50, 100 and 200 mg/L). After 24 hours, the concentrations of IFN-α, IL-6, TNF-α were detected with ELISA. Five days later, the cultured cells were collected and analyzed for immotype, morphology and ultramicrostructure.
RESULTSThe level of IFN-α, IL-6, TNF-α was significantly higher in samples from CML remission group than that in untreated pDCs, and newly diagnosed pDC (P < 0.05) or untreated group. APS could promote more pDCs differentiating to dendritic cells (DCs) in CML remission group than in untreated-pDCs in a dose-dependant manner (P < 0.05).
CONCLUSIONAPS can enhance the immune function of pDCs, promote differentiation and maturation of pDCs from CML patients.
