A comparison of NK cell subsets between rhG-CSF mobilized peripheral blood grafts and bone marrow grafts from healthy donors.
- Author:
Ling-ling XU
1
;
Xiao-jun HUANG
;
Xiang-yu ZHAO
Author Information
- Publication Type:Journal Article
- MeSH: Adolescent; Adult; Blood Donors; Bone Marrow Cells; cytology; Child; Cytokines; secretion; Female; Granulocyte Colony-Stimulating Factor; pharmacology; Hematopoietic Stem Cell Mobilization; methods; Humans; Killer Cells, Natural; cytology; secretion; Male; Middle Aged; Recombinant Proteins; pharmacology; Young Adult
- From: Chinese Journal of Hematology 2011;32(2):79-82
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo analyze the difference in NK cell subsets between recombination human granulocyte colony-stimulating factor (rhG-CSF) mobilized peripheral blood grafts (G-PB) and bone marrow grafts (G-BM) from healthy donors.
METHODSFrom July 2009 to September 2009, G-PB and G-BM from 28 related donors were collected to analyze lymphocytes, NK cells and NK cell secretion of interferon-γ (IFN-γ, NK1), interleukin-13 (IL-13, NK2), transforming growth factor-β (TGF-β) and interleukin-10 (IL-10, NKr) by flow cytometry.
RESULTSThe percentage of lymphocytes in G-PB was significantly higher than that in G-BM (P < 0.01). The proportions of NK cells and NK1, NK2, NKr subsets among lymphocytes were significantly higher in G-BM than in G-PB (P < 0.05), and so were the percentage of NK2, NKr cells among NK cells (P < 0.01), but no significant difference in the percentage of NK1 cells among NK cells between G-PB and G-BM. The ratios of IL-13 and IFN-γ, of TGF-β and IFN-γ, or of IL-10 and IFN-γ in G-BM were significantly higher than those in G-PB (P = 0.010, 0.002, or 0.000, respectively).
CONCLUSIONThe increased proportion of NK2 and NKr in G-BM might be helpful to explain the lower immunoreactivity of G-BM than that of G-PB, although the proportion of NK1 in G-BM and G-PB is similar.
