Ginsenoside-Ro enhances cell proliferation and modulates Th1/Th2 cytokines production in murine splenocytes.
- Author:
Jun-li YU
1
;
De-qiang DOU
;
Xiao-hong CHEN
;
Hong-zhen YANG
;
Xiao-yan HU
;
Gui-fang CHENG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cell Proliferation; drug effects; Ginsenosides; isolation & purification; pharmacology; Interferon-gamma; biosynthesis; genetics; Interleukin-2; metabolism; Interleukin-4; biosynthesis; genetics; Male; Mice; Mice, Inbred BALB C; Panax; chemistry; Plants, Medicinal; chemistry; RNA, Messenger; biosynthesis; genetics; Spleen; cytology; metabolism
- From: Acta Pharmaceutica Sinica 2005;40(4):332-336
- CountryChina
- Language:English
-
Abstract:
AIMTo study the effects of ginsenoside-Ro on cell proliferation and cytokine production in murine splenocytes.
METHODSThe effect of ginsenoside-Ro on murine splenocytes proliferation was studied using [3H] thymidine incorporation assay. Effects of ginsenoside-Ro on the production of cytokines interleukin-2 (IL-2), interferon-gamma (IFN-gamma) and interleukin-4 (IL-4) from murine splenocytes were detected by ELISA method. Effects of ginsenoside-Ro on mRNA level of Th1 cytokine IFN-gamma and Th2 cytokine IL-4 were evaluated by reverse transcription polymerase chain reaction (RT-PCR) analysis.
RESULTSGinsenoside-Ro showed no mitogenic effect on unstimulated murine splenocytes. It enhanced the proliferation of Con A-induced murine splenocytes and the production of IL-2 at concentrations of 1-10 micromol x L(-1). Moreover, ginsenoside-Ro increased the production and expression of Th2 cytokine IL-4 and decreased the production and expression of Th1 cytokine IFN-gamma in Con A-induced murine splenocytes at concentrations of 2-10 micromol x L(-1).
CONCLUSIONGinsenoside-Ro showed immunomodulatory effects by regulating the production and expression of Th1/Th2 cytokines in murine splenocytes.
