Determination of perfluoropropane in canine whole blood by purge and trap concentrator-GC-MS.
- Author:
Li YANG
1
;
Zhi-liang CHEN
;
Xin-ya WANG
Author Information
- Publication Type:Journal Article
- MeSH: Albumins; Animals; Area Under Curve; Capsules; Dogs; Fluorocarbons; administration & dosage; blood; pharmacokinetics; Gas Chromatography-Mass Spectrometry; methods; Injections, Intravenous
- From: Acta Pharmaceutica Sinica 2005;40(4):358-360
- CountryChina
- Language:Chinese
-
Abstract:
AIMTo develop a method for direct determination of perfluoropropane in canine whole blood and to study its pharmacokinetics after a suspension of perfluoropropane-containing albumin microcapsules was administered intravenously.
METHODSPerfluoropropane-containing albumin microcapsule suspension was administered intravenously to anesthetized canines at the dosage of 0.6 mL x kg(-1). Whole blood samples were collected and added directly into the purging glass tube in Tekmar 3000 Purge and Trap Concentrator coupled with a GC-MS for the determination of perfluoropropane. The pharmacokinetic parameters of perfluoropropane were calculated by non-compartment model statistics.
RESULTSThe linear range was 0.0168-4.03 mg x L(-1). The main pharmacokinetic parameters of perfluoropropane was obtained as follows: mean residence time (MRT) was (63 +/- 5) s, T1/2 was (44 +/- 4) s, Tmax was 30 s, Cmax was (2.20 +/- 0.20) mg x L(-1), AUC0-infinity was (96 +/- 11) mg x s x L(-1).
CONCLUSIONThe method is sensitive, specific and simple. It can be used to determine fluorocarbon contained in microcapsule ultrasound contrast agents for studying its pharmacokinetics.
