Tissue distribution of Aconitum alkaloids extracted from Radix aconiti preparata after oral administration to rats.
- Author:
Wen-Dong LI
1
;
Chen MA
Author Information
- Publication Type:Journal Article
- MeSH: Aconitine; analogs & derivatives; metabolism; Aconitum; chemistry; Administration, Oral; Animals; Drugs, Chinese Herbal; administration & dosage; isolation & purification; pharmacokinetics; Kidney; metabolism; Liver; metabolism; Male; Myocardium; metabolism; Plant Roots; chemistry; Plants, Medicinal; chemistry; Rats; Rats, Sprague-Dawley; Spleen; metabolism; Tissue Distribution
- From: Acta Pharmaceutica Sinica 2005;40(6):539-543
- CountryChina
- Language:English
-
Abstract:
AIMTo develop an HPLC method for the determination of Aconitum alkaloids extracted from Radix aconiti preparata in rats.
METHODSWaters 2690@996 PAD system was used. The analytical column was a Halsil 100 C18 column (250 mm x 4.6 mm ID, 5 microm). The mobile phase was water, methanol and diethyl amine at the ratio of 75:25:0.1. The flow rate was 0.9 mL.min(-1). The wavelength of the detector was 240 nm.
RESULTSThe linear ranges of aconitine in the heart, spleen, lung and kidney were 0.4-100 microg.mL(-1), the correlation coefficients were 0.9972, 0.9986, 0.9993 and 0.9994, respectively. The linear range of aconitine in liver was 2-200 microg.mL(-1) and the correlation coefficient was 0.9990. The linear ranges of hypaconitine in heart, liver, spleen, lung, kidney, brain and spinal cord were 5-100 microg.mL(-1), the correlation coefficients were 0.9994, 0.9997, 0.9998, 0.9984, 0.9998, 0.9998 and 0.9997, respectively. Detection limits (S/N = 3) of aconitine and hypaconitine were 0.4 microg.mL(-1). The recoveries of aconitine and hypaconitine ranged from 88.7% to 102.2% and 86.5% to 101.3%, respectively, and the RSD of precision of aconitine and hypaconitine was 10%.
CONCLUSIONIt appears to be an accurate and effective method that can offer reference basis for in toxication of Radix aconiti preparata clinically.
