Determination of peimine and peiminine in Fritillaria thunbergii by HPLC-ELSD.
- Author:
Yan XUE
1
;
Hao-Liang GU
Author Information
- Publication Type:Journal Article
- MeSH: Cevanes; analysis; Chromatography, High Pressure Liquid; methods; Fritillaria; chemistry; classification; Plant Roots; chemistry; Plants, Medicinal; chemistry; Quality Control; Reproducibility of Results
- From: Acta Pharmaceutica Sinica 2005;40(6):550-552
- CountryChina
- Language:Chinese
-
Abstract:
AIMTo determine peimine and peiminine in Fritillaria drug simultaneously by RP-HPLC-ELSD.
METHODSHPLC was carried out with a Waters Alliance, Model 2690, equipped with XTerra RP18 column (150 mm x 3.9 mm ID, 5 microm) and evaporated light scattering detector. The mobile phase (acetonitrile-10 mmol.L(-1) NH4HCO3 adjusted to pH 10.10 by ammonia solution) was eluted in gradient mode.
RESULTSThe recoveries of peimine and peiminine were 98.96% (n = 4), with RSD 1.01% and 98.40% (n = 4), with RSD 2.63%, respectively.
CONCLUSIONThe method is simple, sensitive and reliable. It can be used for quantitative determination of Fritillaria drug.
