Pharmacokinetics of breviscapine and its beta-cyclodextrin complex in rats.
- Author:
Hai-Yan ZHANG
1
;
Qi-Neng PING
;
Jian-Xin GUO
;
Feng CAO
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Area Under Curve; Chromatography, High Pressure Liquid; methods; Drug Compounding; Erigeron; chemistry; Flavonoids; blood; isolation & purification; pharmacokinetics; Male; Plants, Medicinal; chemistry; Random Allocation; Rats; Rats, Sprague-Dawley; beta-Cyclodextrins; blood; pharmacokinetics
- From: Acta Pharmaceutica Sinica 2005;40(6):563-567
- CountryChina
- Language:Chinese
-
Abstract:
AIMTo establish RP-HPLC method for determination of plasma scutellarin concentration and study of the pharmacokinetic behavior of scutellarin in rat after ig administration of breviscapine and its beta-cyclodextrin complex (breviscapine-beta-CD).
METHODSMobile phase composed of methanol and acetate buffer. The column was Shim-pack C18. Twelve rats randomized into 2 groups were separately given breviscapine and breviscapine-beta-CD at single dose of 10.8 mg.kg(-1). Drug in plasma was extracted and determined by HPLC. The pharmacokinetic parameters were calculated by 3P97 software.
RESULTSLinearity was obtained over the range of 10-400 ng.mL(-1). The recovery was 95.32%-98.81%. C(max) and AUC(0-12 h) of breviscapine were (154 +/- 18) ng.mL(-1) and (710 +/- 126) ng.h.mL(-1). For breviscapine-beta-CD, C(max) and AUC(0-12 h) were (328 +/- 31) ng.mL(-1) and (1,093 +/- 200) ng.h.mL(-1), respectively. There were significant differences of AUC(0-12 h) between breviscapine and breviscapine-beta-CD (P < 0.01).
CONCLUSIONThe assay method was suitable for the determination of scutellarin plasma concentration in rat. Brevescapine-beta-CD showed greater absorption compared with that of brevescapine.
