Preparation of cardiomyocyte-targeting liposomes and their targeting ability in vitro.
- Author:
Yan CHEN
1
;
Ying-Jie DENG
;
Yan-Li HAO
;
Zhen-Yuan WANG
;
Jun SHENG
Author Information
- Publication Type:Journal Article
- MeSH: Adrenergic beta-Antagonists; pharmacology; Animals; Animals, Newborn; Cell Hypoxia; Cells, Cultured; Drug Carriers; Drug Delivery Systems; Fatty Alcohols; chemistry; metabolism; Liposomes; Mice; Molecular Structure; Myocytes, Cardiac; drug effects; metabolism; Propanolamines; pharmacology
- From: Acta Pharmaceutica Sinica 2005;40(6):568-572
- CountryChina
- Language:Chinese
-
Abstract:
AIMTo prepare the cardiomyocyte-targeting liposomes and investigate their cardiomyocyte targetability in vitro.
METHODSLiposomes modified with compound PAC were prepared (PAC-L); The uptake of PAC-L by cardiomyocytes was studied by incubating fluorescence labeled liposomes with cardiomyocytes in vitro and measuring the association of liposomes by a fluorescence spectrophotometer.
RESULTSA high affinity of PAC-L to the cardiomyocytes was observed, the amount of cell uptake of PAC-L by cardiomyocytes was higher than that by nonmyocyte (P < 0.001); The amount of cardiomyocyte uptake of PAC-L on the normoxia condition 2 h was 2.9-fold higher than that of plain-L, and the increase was 5.2-fold when hypoxia occured; The form of liposome uptake changed, the amount of cardiomyocyte uptake of Plain-L by internalization was only 11%, while that of PAC-L was 56%.
CONCLUSIONIt is indicated that PAC-L was a potential drug carrier for targeting to ischemic myocardium.
