Interaction between balofloxacin and DNA and the influence of Mg2+ on the interaction.
- Author:
Wen-Ying ZHONG
1
;
Qi HUANG
;
Dan-Dan WANG
;
Kun-Yi NI
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cattle; DNA; chemistry; Fluoroquinolones; chemistry; Magnesium; chemistry; Spectrometry, Fluorescence; Spectrophotometry, Ultraviolet; Thermodynamics
- From: Acta Pharmaceutica Sinica 2005;40(7):663-667
- CountryChina
- Language:Chinese
-
Abstract:
AIMTo study the binding mode of balofloxacin with DNA and evaluate the influence of Mg2+ on the binding between balofloxacin and DNA.
METHODSFluorescent spectroscopy was used to study the interaction of balofloxacin with DNA and to calculate the thermodynamic constants. UV-Vis spectra, DNA viscosity titration, competition experiment and the effect of dsDNA and ssDNA on the fluorescense intensity were used to identify the binding mode.
RESULTSBalofloxacin interacted with CT-DNA with a quenching constant of (5.43 +/- 0.07) x 10(3) L x mol(-1). The interaction was exothermic with a Van't Hoff enthalply of - 8.03 kJ x mol(-1) x Mg2+ cation could enhance the quenching constant between balofloxacin and DNA.
CONCLUSIONBalofloxacin interacted with CT-DNA in the mode of groove binding and Mg2+ could mediate the binding of balofloxacin to DNA.
