Effects of ferulic acid on E-selectin expression in activated endothelial cell and leukocyte-endothelial cell adhesion.
- Author:
Xiao-lan WANG
1
;
Xiao-hui HU
;
Ming-en LÜ
;
Zhen-lun GU
;
Chang-geng RUAN
Author Information
- Publication Type:Journal Article
- MeSH: Cell Adhesion; drug effects; Cells, Cultured; Coumaric Acids; pharmacology; E-Selectin; biosynthesis; genetics; Endothelial Cells; metabolism; HL-60 Cells; physiology; Humans; RNA, Messenger; biosynthesis; genetics; Umbilical Veins; cytology
- From: Acta Pharmaceutica Sinica 2005;40(5):410-413
- CountryChina
- Language:English
-
Abstract:
AIMTo study the effects of ferulic acid (FA) on E-selectin expression in human umbilical vein endothelial cells (HUVECs) activated by lipopolysaccharide and leukocyte-endothelial cell adhesion.
METHODSThe effects of FA on E-selectin and E-selectin mRNA expression were determined by flow cytometry and reverse transcription polymerase chain reaction. The effect of FA on HL60-HUVEC adhesion was evaluated with the method of staining the cells by Rose Bengal.
RESULTSThe expression of E-selectin and E-selectin mRNA were down regulated by FA (0.62 and 0.41 mmol x L(-1), respectively). HL60 cells adhered to activated HUVECs were also reduced by FA (0.62 and 0.41 mmol x L(-1), respectively).
CONCLUSIONFA can inhibit the expression of E-selectin and E-selectin mRNA and HL60-HUVEC adhesion. This may contribute to its protective effect against ischemia-reperfusion injury.
