Intervention of cetirizine on monocyte chemoattractant protein-1 in cutaneous inflammation.
- Author:
Hong-jie SONG
1
;
Jin-hong HU
;
Jin HUANG
;
Yan-feng XU
;
Li JING
Author Information
- Publication Type:Journal Article
- MeSH: Cell Line; Cells, Cultured; Cetirizine; pharmacology; Chemokine CCL2; biosynthesis; genetics; Dermatitis; metabolism; Dermis; cytology; Fibroblasts; cytology; metabolism; Histamine; pharmacology; Histamine H1 Antagonists, Non-Sedating; pharmacology; Humans; Interferon-gamma; pharmacology; Keratinocytes; cytology; metabolism; RNA, Messenger; biosynthesis; genetics
- From: Acta Pharmaceutica Sinica 2005;40(5):414-417
- CountryChina
- Language:Chinese
-
Abstract:
AIMTo study the intervention of cetirizine on monocyte chemoattractant protein-1 (MCP-1) in different cutaneous inflammation models.
METHODSHistamine and IFN-gamma stimulated dermal fibroblast cells and HaCaT cells to mimic cutaneous inflammation. Expression of MCP-1 was assessed by means of RT-PCR and ELISA.
RESULTSCompared with the control group of dermal fibroblast (DF) cells and HaCaT cells, MCP-1 mRNA was significantly upregulated by histamine (10 micromol x L(-1)) and IFN-gamma (20 ng x mL(-1)). The protein secretions of MCP-1 were increased 3.5 fold and 8.4 fold in DF cells, respectively. The similar tendency was observed in HaCaT cells. The enhancing effects of histamine and IFN-gamma on MCP-1 protein production were significantly inhibited by cetirizine (1 and 10 micromol x L(-1)) in DF and HaCaT cells.
CONCLUSIONCetirizine may exert the anti-inflammatory effect of skin via inhibiting MCP-1 expression.
