Effect of salidroside on mitochondria injury induced by sodium azide.
- Author:
Li-li CAO
1
;
Guan-hua DU
;
Min-wei WANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Brain Neoplasms; pathology; physiopathology; Cell Line, Tumor; Cell Survival; Glucosides; isolation & purification; pharmacology; Humans; Male; Membrane Potentials; drug effects; Mitochondria; drug effects; metabolism; Neuroblastoma; pathology; physiopathology; Oxidation-Reduction; drug effects; Phenols; isolation & purification; pharmacology; Plants, Medicinal; chemistry; Rats; Rats, Sprague-Dawley; Rhodiola; chemistry; Sodium Azide
- From: Acta Pharmaceutica Sinica 2005;40(8):700-704
- CountryChina
- Language:Chinese
-
Abstract:
AIMTo study the protective effect of salidroside on mitochondria injury induced by sodium azide.
METHODSHuman neuroblastoma SH-SY5Y cells were exposed to sodium azide with different concentration of salidroside, then cell viability was measured by thiazolyl blue (MTT) method and mitochondrial membrane potential (MMP) was detected by JC-1 method. Protective effect of salidroside against disfunction of mitochondria induced by sodium azide was detected by resazurin method.
RESULTSAfter exposing to 64 mmol x L(-1) sodium azide for 4 hours, cell viability and MMP of SH-SY5Y cells significantly decreased. When pretreated with salidroside, the cell damage was greatly reduced and the mitochondrial membrane potential was maintained. Furthermore, salidroside can protect function of rat brain mitochondria against damage induced by sodium azide.
CONCLUSIONSalidroside was demonstrated to play an important role in improving the function of mitochondria.
