Effect of ginkgolide B on the production of NO, IL-6 and RANTES from astrocytes.
- Author:
Shan-ying PENG
1
;
Wen-hui LIAO
;
Zhen-gui NIE
;
Yang LIU
;
Lin WANG
;
Feng WANG
;
Wen-jie WANG
Author Information
1. Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Astrocytes;
cytology;
metabolism;
Cell Line, Tumor;
Cells, Cultured;
Chemokine CCL5;
genetics;
metabolism;
Dose-Response Relationship, Drug;
Ginkgolides;
administration & dosage;
pharmacology;
Glioblastoma;
metabolism;
pathology;
Humans;
Interleukin-1beta;
Interleukin-6;
genetics;
secretion;
Lactones;
administration & dosage;
pharmacology;
Lipopolysaccharides;
Male;
Mice;
Mice, Inbred C57BL;
Nitric Oxide;
metabolism;
Platelet Activating Factor;
antagonists & inhibitors;
RNA, Messenger;
metabolism;
Rats;
Rats, Wistar
- From:
Acta Pharmaceutica Sinica
2010;45(9):1103-1108
- CountryChina
- Language:Chinese
-
Abstract:
This study is to explore the effect of ginkgolide B (BN52021) on the production of nitric oxide (NO), interleukin (IL)-6 and regulated upon activation normal T cell expressed and secreted (RANTES) from astrocytes induced by stimulators. Primary cultured rat astrocytes were stimulated with lipopolysaccharides (LPS), the production of NO was assayed using Griess reaction; U251 cells were stimulated with IL-1 beta, the contents of IL-6 and RANTES in the supernatant were measured using ELISA. The mRNA expressions of IL-6 and RANTES were detected using RT-PCR. LPS (10 ng mL(-1) to 10 microg mL(-1)) could stimulate rat astrocytes to produce NO in a dose-dependent manner. Ginkgolide B at the concentrations of 0.1-10 micromol L(-1) were shown to decrease NO production significantly. IL-1 beta could induce the mRNA expression and protein secretion of IL-6 from U251 cells, as well as RANTES. Ginkgolide B at concentrations of 0.1-10 micromol L(-1) were shown to inhibit RANTES secretion, and to inhibit mRNA expression of IL-6 and RANTES at concentration of 10 micromol L(-1). Ginkgolide B has inhibitory effect on the production of NO, IL-6 and RANTES from astrocytes treated with inflammatory stimulators.
