Development of a new HPLC technique for analyzing monosaccharide composition and its application in the quality control of Silybum marianum polysaccharide.
- Author:
Ling-Min TIAN
1
;
Xue-Mei QIU
;
Zi-Jing PAN
;
You LÜ
;
Xing-Bin YANG
Author Information
1. Key Laboratory of Ministry of Education for Medicinal Plant Resource and Natural Pharmaceutical Chemistry, College of Life Sciences, Shaanxi Normal University, Xi 'an 710062, China.
- Publication Type:Journal Article
- MeSH:
Antipyrine;
analogs & derivatives;
chemistry;
Arabinose;
analysis;
Chromatography, High Pressure Liquid;
methods;
Galactose;
analysis;
Glucose;
analysis;
Glucuronic Acid;
analysis;
Hexuronic Acids;
analysis;
Mannose;
analysis;
Milk Thistle;
chemistry;
Monosaccharides;
analysis;
Plants, Medicinal;
chemistry;
Polysaccharides;
chemistry;
isolation & purification;
Quality Control;
Rhamnose;
analysis;
Seeds;
chemistry;
Spectrophotometry, Ultraviolet;
methods;
Xylose;
analysis
- From:
Acta Pharmaceutica Sinica
2010;45(4):498-504
- CountryChina
- Language:Chinese
-
Abstract:
A new HPLC-UV technique for the separation and analysis of 10 monosaccharides achieved within 13.5 min using 1-phenyl-3-methyl-5-pyrazolone (PMP) as the labelling molecule of the reductive monosaccharides has been established by combining common high performance liquid chromatography-UV and C18 column. The established technique was applied to the quantification of the monosaccharide components in extract of Silybum marianum. The results showed that the tested 10 monosaccharides as PMP derivatives were baseline separated under the HPLC conditions proposed. It was confirmed that Silybum marianum extract was composed of mannose, rhamnose, glucuronic acid, galacturonic acid, glucose, xylose, galactose and arabinose with the molar ratio of 0.66:0.84:0.58:1.0:1.6:0.69:2.7:4.8. Quantitative recoveries of the compositional monosaccharides separated from the extract were in the range of 92.4%-104.0%, and the RSD values fell within 0.68%-3.81%. The results demonstrated that the proposed HPLC method was simple, rapid, convenient, and precise, and it was applicable to the analysis of the compositional monosaccharides of Silybum marianum extract.
