Transcriptome characterization for Salvia miltiorrhiza using 454 GS FLX.
- Author:
Ying LI
1
;
Chao SUN
;
Hong-Mei LUO
;
Xi-Wen LI
;
Yun-Yun NIU
;
Shi-Lin CHEN
Author Information
1. Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, China.
- Publication Type:Journal Article
- MeSH:
Alkenes;
Cytochrome P-450 Enzyme System;
genetics;
DNA, Plant;
genetics;
Databases, Nucleic Acid;
Diterpenes, Abietane;
biosynthesis;
genetics;
Expressed Sequence Tags;
Gene Expression Profiling;
methods;
Genes, Plant;
genetics;
High-Throughput Nucleotide Sequencing;
methods;
Microsatellite Repeats;
genetics;
Plant Roots;
genetics;
Plants, Medicinal;
genetics;
Polyphenols;
biosynthesis;
genetics;
Salvia miltiorrhiza;
genetics;
Sequence Analysis, DNA;
Transcriptome;
genetics
- From:
Acta Pharmaceutica Sinica
2010;45(4):524-529
- CountryChina
- Language:Chinese
-
Abstract:
To investigate the profile of gene expression in Salvia miltiorrhiza and elucidate its functional gene, 454 GS FLX platform and Titanium regent were used to produce a substantial expressed sequence tags (ESTs) dataset from the root of S. miltiorrhiza. A total of 46 722 ESTs with an average read length of 414 bp were generated. 454 ESTs were combined with the S. miltiorrhiza ESTs from GenBank. These ESTs were assembled into 18 235 unigenes. Of these unigenes, 454 sequencing identified 13 980 novel unigenes. 73% of these unigenes (13 308) were annotated using BLAST searches (E-value < or = 1e-5) against the SwissProt, KEGG TAIR, Nr and Nt databases. Twenty-seven unigenes (encoding 15 enzymes) were found to be involved in tanshinones biosynthesis, and 29 unigenes (encoding 11 enzymes) involved in phenolic acids biosynthesis. Seventy putative genes were found to encode cytochromes P450 and 577 putative transcription factor genes. Data presented in this study will constitute an important resource for the scientific community that is interested in the molecular genetics and functional genomics of S. miltiorrhiza.
