Study on the anti-tumor effect of paclitaxel mixed micelle by using in vivo optical imaging technique.
- Author:
Wen TAI
1
;
Min-Min SUN
;
Nan LIU
;
Zhi-Qi HUANG
;
Shang-Hai NIE
;
Yan-Li HAO
;
Xiao-Ning ZHANG
Author Information
1. Medical School of Tsinghua University, Beijing 100084, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Antineoplastic Agents, Phytogenic;
administration & dosage;
pharmacology;
therapeutic use;
Breast Neoplasms;
drug therapy;
pathology;
Cell Line, Tumor;
Female;
Humans;
Luminescent Measurements;
Male;
Melanoma, Experimental;
drug therapy;
pathology;
Mice;
Mice, Inbred C57BL;
Mice, Nude;
Micelles;
Neoplasm Transplantation;
Paclitaxel;
administration & dosage;
pharmacology;
therapeutic use;
Particle Size;
Tumor Burden;
drug effects
- From:
Acta Pharmaceutica Sinica
2010;45(4):530-534
- CountryChina
- Language:Chinese
-
Abstract:
In vivo tumor imaging technique method based on bioluminescence principle was established to evaluate the anti-tumor effect of paclitaxel mixed micelle (PMM). MDA-MB-231 tumor cells with luciferase reporter vectors were firstly implanted into nude mice, and subsequently the luciferase substrate was regularly injected during intraperitoneal administration of PMM. Then the tumor size, growth and the intensity of light signals were monitored with in vivo imaging technique. The method of luciferase tumor in vivo imaging could be real-time, reliable and exact in labeling and reflecting the growth of tumors, and the observed results were consistent with that by conventional method, so it would be a feasible approach to study anti-tumor effect of drugs. The anti-tumor effect of paclitaxel mixed micelle was observed by this method, and the results showed that this formulation could inhibit growth of tumor, and the anti-tumor rate of it was about 85%.
