PEGylation of polyamidoamine dendrimer and the properties for gene vectors.
- Author:
Chi WANG
1
;
Shi-Rong PAN
;
Hong-Mei WU
;
Yu-Ting WEN
;
Xin ZENG
;
Min FENG
Author Information
1. The First Affiliated Hospital, Sun Yet-sen University, Guangzhou 510080, China.
- Publication Type:Journal Article
- MeSH:
Carcinoma, Hepatocellular;
pathology;
Cell Line, Tumor;
Cell Survival;
drug effects;
DNA;
chemistry;
pharmacology;
Dendrimers;
chemical synthesis;
pharmacology;
Gene Transfer Techniques;
Genetic Vectors;
Humans;
Isocyanates;
chemistry;
Liver Neoplasms;
pathology;
Particle Size;
Polyamines;
chemistry;
Polyethylene Glycols;
chemical synthesis;
chemistry;
pharmacology;
Transfection
- From:
Acta Pharmaceutica Sinica
2011;46(1):102-108
- CountryChina
- Language:Chinese
-
Abstract:
Polyamidoamine-polyethylene glycol (PAMAM-PEG) copolymers were synthesized using IPDI as coupling reagent by two-step method. The copolymers were characterized by IR spectrum and 1H NMR spectrum, and the PEG conjugating ratios of the copolymers were calculated equal to 10% and 30% separately. MTT assay indicated that after PEGylation a lower cytotoxicity of the copolymers could be found, and with increasing PEG conjugating ratio the cytotoxicity decreased obviously. Agarose gel retardation assay demonstrated that PAMAM-PEG copolymers could be combined with DNA and PAMAM-PEG/DNA complexes were prepared by self-assembly. DLS measurement showed that when N/P > or = 50, the particle size of copolymer/ gene complexes was in a range of 150-200 nm, and the zeta potential was in a range of 10-25 mV. In vitro gene transfection illustrated that when N/P < or = 50, the gene transfection efficiency of PAMAM-PEG copolymers was a little less than that of PAMAM-G5, but the transfection efficiency can be raised by increasing N/P ratio or transfection time. Considering both cytotoxicity and transfection efficiency aspects PAMAM-PEG-13 was more effect than PAMAM-PEG-39 in PEGylation.
