Induction of HSF1 expression and sporadic colorectal cancer.
- Author:
Yong-ming FANG
1
;
Qi DONG
;
Hui CEN
;
Xiao-ping TANG
;
Shu ZHENG
Author Information
- Publication Type:Journal Article
- MeSH: Colorectal Neoplasms; genetics; metabolism; DNA-Binding Proteins; biosynthesis; genetics; Gene Expression Regulation, Neoplastic; Heat Shock Transcription Factors; Humans; Intracellular Signaling Peptides and Proteins; genetics; Nerve Tissue Proteins; biosynthesis; genetics; Reverse Transcriptase Polymerase Chain Reaction; Signal Transduction; physiology; Transcription Factors; Tumor Suppressor Proteins; biosynthesis; genetics
- From: Journal of Zhejiang University. Medical sciences 2004;33(5):390-394
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the activation of the signal transduction pathways related with the carcinogenesis of sporadic colon cancers.
METHODSA gene microarray monitoring activation of 8 signal transduction pathways (PathwayFinder GEArray) was used to screen the differentially expressed genes between colorectal cancer and normal colon tissue. The differentially expressed genes were further analyzed by RT-PCR, using RNA extracted from cancer tissue and matched normal colon mucosa of 35 patients with colorectal cancer.
RESULTSThe expression of hsf1, hsf27 and inos was increased in colon cancer compared with normal colon mucosa using PathwayFinder GEArray. The RT-PCR results showed that the expression of hsf1 was detected in 86% of patients(30/35)and the expression of inos detected in 63% patients(22/35).
CONCLUSIONHsf1 induces heat shock stress signaling pathway, which might play a role in the carcinogenesis of sporadic colorectal cancer.
