Expression of telomere repeat binding factor 1 (TRF1) protein in kidney cancer.

Author: Ji-min SHI ¹ ; Wei DING ; He HUANG ; Zhi-gen ZHANG ; Li-dan TANG ; Mao-fang LIN
Author Information

1. The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou 310003, China.
Publication Type:Journal Article
MeSH: Adenocarcinoma, Clear Cell; metabolism; Adult; Aged; Aged, 80 and over; Female; Humans; Kidney; metabolism; Kidney Neoplasms; metabolism; Male; Middle Aged; RNA, Messenger; biosynthesis; genetics; Telomeric Repeat Binding Protein 1; biosynthesis; genetics
From: Journal of Zhejiang University. Medical sciences 2004;33(6):496-508
CountryChina
Language:Chinese
Abstract:
OBJECTIVETo investigate the expression levels of telomere repeat binding factor 1(TRF1) protein in normal kidney tissue and kidney cancer.
METHODSSpecimens of kidney cancer and pericancerous tissues were collected from 32 cases of renal carcinoma. A quantitative Western blotting technique was developed using TRF1 monoclonal antibody to determine the expression level of TRF1 protein in total protein extracts from tissue specimens.
RESULTSThe expression level of TRF1 protein was higher in normal kidney tissues (3.611 +/-1.922 microg/microl) than that of cancer tissues (2.428 +/-1.352 microg/microl) (t=5.776, P<0.01).
CONCLUSIONThe expression level of TRF1 protein is significantly reduced in kidney cancer and the level is negatively correlated with malignant degree of the cancer.